Centrifuging infectious materials or human samples

Avoid the use of celluloid tubes with biohazards. If celluloid tubes must be used, an appropriate chemical disinfectant must be used to decontaminate them. 2 Always use sealed safety cups, safety buckets, or sealed rotors with O rings as secondary containment. 3 Always wear gloves when handling tubes or rotors. 4 Place a biohazard label on the centrifuge. 5 Fill centrifuge tubes, load into rotors, remove from rotors, and open tubes within a biological safety cabinet whenever possible. 6 Wipe exterior of tubes or bottles with disinfectant prior to loading into rotor or bucket. Seal rotor or bucket, remove outer gloves, and transport to the centrifuge. 7 Wait at least 10 minutes after the run to allow aerosols to settle before opening the centrifuge. Check for possible spills or leaks. For spills of infectious materials transmitted by inhalation, see Emergency Procedures below.