Safe Procedures for Centrifugation
Precautions while using Centrifuge
Safe Procedures for Centrifugation
Before centrifugation 1 Train each operator on proper operating procedures, review the user manual. 2 Use only rotors compatible with the centrifuge. Check the expiration date for ultracentrifuge rotors.3 Check tubes, bottles, and rotors for cracks and deformities before each use. 4 Make sure that the rotor, tubes, and spindle are dry and clean. 5 Examine O rings and replace if worn, cracked, or missing. 6 Never overfill centrifuge tubes (don t exceed ? full). 7 Always cap tubes before centrifugation. Always balance8 buckets, tubes, and rotors properly.9 Check that the rotor is seated on the drive correctly, close the lid on the centrifuge, and secure it.10 When using swinging bucket rotors, make sure that all buckets are hooked correctly and move freely.
High speed centrifuges
A laboratory centrifuge
Know your rotors and maintain a log book
The work surface must be level and firm
Interlocks
Do not open the lid while the rotor is moving
Specific guidance
Wear a face shield and or safety goggles
Inner safety lids
Centrifuge Maintenance
Discard any centrifuge tubes that have cracks in them
Safe Procedures for Centrifugation
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