precautions while using centrifuge

centrifuge pre cooled state, the centrifuge lid must be closed, after centrifugation turned out to be inverted in the experimental stage, dry the cavity more than water, the centrifuge lid is open. II. turned to when the transfer head covered in the pre cooling can be placed in the centrifuge platform, or placed in the experimental stage, do not tighten the float is not turned on, because once the errors start, turn skull will fly out, causing the accident! III. turn after tightening bonnet must use the touch of a finger and turned to whether the gap between the switch cover and, if re opened the gap to tighten the screw until the confirmation before starting the centrifuge seamless. IV. In the centrifuge process, the operator shall not leave the centrifuge chamber, in the event of unusual circumstances the operator can not turn off the power POWER, according to STOP. Fill in the pre cooling centrifuge before use records.

Discontinue use of any centrifuge that does not have a lid and do not retrofit the unit with a lid that does not have a latch. All personnel should follow universal precautions when performing centrifugation and other functions that may expose workers to splashed blood or body fluids. These precautions include wearing gloves, facial protection (e.g., shields), gowns or laboratory coats, and plastic aprons; these are described in detail in the National Committee for Clinical Laboratory Standards (NCCLS) tentative guideline and the Occupational Safety and Health Administration s (OSHA) bloodborne pathogens standard.

Discontinue use of any centrifuge that does not have a latch; the latch keeps the centrifuge lid closed in the event of tube breakage or other problems while the centrifuge is operating. All such units should be replaced or modified to include a latch; contact the manufacturer for information.

If available, purchase centrifuges that have lids with interlocks to prevent the user from opening the lid while the rotor is spinning. Centrifuge rotors are made to withstand these extreme centrifugal forces (i.e., they re designed to give enough centripetal force to keep the rotor together as it spins), but it s better to be prepared in case something fails. It may be fascinating to watch the tubes spin around in the centrifuge, but don t make it a habit. Turn the unit on and keep your distance until well after the timer shuts off the motor this is just a basic safety rule for all centrifuges.

When possible, purchase centrifuges that have inner safety lids for the buckets or rotor. If the units being purchased allow the outer lid to be opened while the rotor is spinning at low speeds, be sure they have protective inner lids.

Ensure that the centrifuge tubes are properly balanced and that the speed and tube length are in accordance with the tube and the centrifuge manufacturer s recommendations. Do not use tubes that are not properly sized for the rotor. If using a swinging bucket rotor, ensure that the tubes are placed in accordance with the manufacturer s instructions; long tubes (e.g., greater than 100 mm) placed in the corner tube holders closest to the rotor shaft may break when the rotor buckets swing out.

For centrifuges with swinging bucket rotors, fasten a protective inner safety lid (if available for your model centrifuge) onto the bucket; for those with fixed angle rotors, fasten an inner safety lid to the rotor before centrifugation. Ensure that the safety lid is properly sealed and positively locked into place.

During preventive maintenance, ensure that the nut that secures the rotor to the shaft is tight (check the manufacturer s instructions).Clean and disinfect all centrifuges regularly according to the manufacturer s recommendations. Contact the manufacturer for guidance if the operator s manual does not specify cleaning or disinfecting agents. Prolonged contact with some disinfectants (e.g., 10% sodium hypochlorite) may damage the rotor and other centrifuge components; be sure that such solutions are removed by rinsing well with water.

Centrifugation may present two serious hazards: mechanical failure and dispersion of aerosols. This fact sheet describes safety and maintenance procedures to minimize centrifuge hazards. Use of centrifuges by new users must be closely supervised by the employee responsible for each centrifuge until he/she is satisfied that unsupervised usage of the instrument is safe. All users must be familiar with the safety procedures for all of the centrifuges that they use, including proper load selection, balancing and maintenance of rotor where necessary.

Before centrifugation 1 Train each operator on proper operating procedures, review the user manual. 2 Use only rotors compatible with the centrifuge. Check the expiration date for ultracentrifuge rotors.3 Check tubes, bottles, and rotors for cracks and deformities before each use. 4 Make sure that the rotor, tubes, and spindle are dry and clean. 5 Examine O rings and replace if worn, cracked, or missing. 6 Never overfill centrifuge tubes (don t exceed ? full). 7 Always cap tubes before centrifugation. Always balance8 buckets, tubes, and rotors properly.9 Check that the rotor is seated on the drive correctly, close the lid on the centrifuge, and secure it.10 When using swinging bucket rotors, make sure that all buckets are hooked correctly and move freely.