CONDENSER DIAPHRAGM ADJUSTMENT

USING THE CONDENSER FOCUS ADJUSTMENT KNOB THAT IS LOCATED ON THE LEFT SIDE OF YOUR MICROSCOPE JUST BELOW THE STAGE, MOVE THE CONDENSER UP TOWARD THE STAGE AS FAR AS IT WILL GO. Optical and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the subsequent collection of this scattered radiation or another signal in order to create an image. This process may be carried out by wide field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning of a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy).